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Thermal restraint of a bacterial exopolysaccharide of shallow vent origin

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Publication
https://doi.org/10.1016/j.ijbiomac.2018.03.160
Domain
Life & Health Sciences
Fellow
Salvatore Magazù

International Journal of Biological Macromolecules, Elsevier, 2018, 114, pp.649-655.

Maria Teresa Caccamo1,4, Vincenzo Zammuto5,6, Concetta Gugliandolo5, Claire Madeleine-Perdrillat3, Antonio Spano5, Salvatore Magazu1,2,3,4

 

1 Dept of Mathematical and Informatics Sciences, Physical Sciences and Earth Sciences of Messina University, Viale Ferdinando Stagno D' Alcontres 31, 98166 Messina, Italy

2 Le Studium Loire Valley Institute for Advanced Studies, 1 rue Dupanloup 45000, Orléans, France

3 Centre de Biophysique Moleculaire (CBM)-CNRS UPR 4301 du CNRS, rue Charles Sadron, 45071 Orleans CEDEX 2 France; Laboratoire Interfaces, Confinement, Matériaux et Nanostructures (ICMN) - UMR 7374 CNRS - Université d'Orléans, 1b rue de la Férollerie, CS 40059, 45071 Orléans cedex 2, France

4 Istituto Nazionale di Alta Matematica “F. Severi” – INDAM – Gruppo Nazionale per la Fisica Matematica – GNFMV, Piazzale Aldo Moro, 5, 00185 Roma, Italy

5 Dept. of Chemical, Biological, Pharmaceutical and Environmental Sciences, Messina University, Viale Ferdinando Stagno D' Alcontres 31, 98166 Messina, Italy

6 Dept. of Earth and Marine Sciences, University of Palermo, Via Archirafi, 22, 90123 Palermo, Italy

Abstract

To dynamically characterize the thermal properties of the fructose-rich exopolysaccharide (EPS1-T14), produced by the marine thermophilic Bacillus licheniformis T14, the Attenuated Total Reflectance Fourier Transform Infra-Red spectroscopy was coupled to variable temperature ranging from ambient to 80 °C.

The spectra were analyzed by the following innovative mathematical tools: i) non-ideal spectral deviation, ii) OH-stretching band frequency center shift, iii) spectral distance, and iv) wavelet cross-correlation analysis.

The thermal restraint analysis revealed that the whole EPS1-T14 system possessed high stability until 80 °C, and suggested that fucose was mainly involved in the EPS1-T14 thermal stability, whereas glucose was responsible for its molecular flexibility.

Our results provide novel insights into the thermal stability properties of the whole EPS1-T14 and into the role of its main monosaccharidic units. As a new biopolymer, the thermostable EPS1-T14 could be used in traditional biotechnology fields and in new biomedical areas, as nanocarriers, requiring high temperature processes.

Keywords

Extremophiles
Thermal analysis
Attenuated-Total-Reflectance Infra-Red spectroscopy
Spectral distance
Wavelet cross-correlation analysis
Published by

Elsevier