Niche, Nutrition, Cancer & Oxidative metabolism (N2COX)

Our research project multidisciplinary single team project focused on lipids acting in/on tumor niches, more particularly on energy/oxidative metabolism and calcium signaling, able to influence dysplasia, tumor progression and sensitivity of tumors to anti-cancer drugs. The objective of our bench to bedside research is to facilitate the transfer of fundamental knowledge to patients developing chemo-or hormone-resistant cancers, and/or metastases and/or cancer-induced cachexia.

Research topics:

• Oncology/Hematological malignancies: lipids in tumor microenvironment and prevention
• Impact of lipids on redox/energy metabolism of cancer cells in their niches
• Chemo/hormono sensitization of tumors to anticancer treatments
• Signal transduction, biology of cancer cells
• Role, mechanism of action of ion channels and transporters, calcium homeostasis
• Clinical nutrition: energy / nitrogen metabolism, cachexia, mitochondrial bioenergetics

Equipment and technology:

• Energy/oxidative metabolism (Avatar® systems, Seahorse®, Omnilog®, Oroboros®) 
• Microspectrofluorimetry/imaging and fluorescent probes
• Chemistry technical facility (analytical and synthesis)
• Flow cytometry and cell sorting
• Electrophysiology (microelectrode, patch-clamp)
• RNA-seq, scRNA-seq, methylome, RT-qPCR, plasmid transfections
• CPG, HPTLC
• Fluorescence microscopes/macroscope
• Time lapse
• Histopathology technical facility
• Indirect calorimetry
• Natural isotopic abundances by EA-IRMS
• Body composition by multi-frequency impedance and biphotonic absorptiometry